The relative expression levels of CD148 and CD180 on clonal B cells and CD148/CD180 median fluorescence intensity ratios are useful in the characterization of mature B cell lymphoid neoplasms infiltrating blood and bone marrow - Results from a single centre pilot study

Arambam Gautam; Sreejesh Sreedharanunni; Man Updesh Singh Sachdeva; Sonia Rana; Dharambir Kashyap; Parveen Bose; Amanjeet Bal; Gaurav Prakash; Pankaj Malhotra; Reena Das; Neelam Varma

doi:10.1111/ijlh.13467

The relative expression levels of CD148 and CD180 on clonal B cells and CD148/CD180 median fluorescence intensity ratios are useful in the characterization of mature B cell lymphoid neoplasms infiltrating blood and bone marrow - Results from a single centre pilot study

Int J Lab Hematol. 2021 Oct;43(5):1123-1131. doi: 10.1111/ijlh.13467. Epub 2021 Jan 17.

Affiliations

¹ Department of Hematology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
² Department of Histopathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
³ Adult Clinical Hematology and Bone Marrow Transplantation Unit, Department of Internal Medicine, Post Graduate Institute of Medical Education and Research, Chandigarh, India.

PMID: 33455071
DOI: 10.1111/ijlh.13467

Abstract

Introduction: The categorization of mature B cell neoplasms (MBN) infiltrating blood and bone marrow are met with difficulties. The inclusion of CD148 and CD180 in the routine flow cytometry/FCM panels has been suggested to refine the diagnosis. We studied the discriminatory ability of CD148 and CD180 median fluorescence intensity(MFI), CD148/CD180 ratio and their expression relative to T cells (CD148^ab/T , CD180^ab/T ), neutrophils (CD148^ab/gr , CD180^ab/gr ) and normal B cells (CD148^ab/n , CD180^ab/n ) in the differentiation of mature B cell neoplasms (MBN) especially non-chronic lymphocytic leukaemia (CLL).

Methodology: The flow cytometric (FCM) expression of CD148 and CD180 was studied prospectively in 102 patients (non-CLL; n = 72); diagnosed by a comprehensive panel of immunophenotypic and cytogenetic studies. The MFI and ratios were statistically compared across MBNs by Mann-Whitney U test. Cut-off values, sensitivity and specificity were calculated for significant parameters by receiver operator characteristic curve.

Results: CD180MFI > 4.35 showed 100% sensitivity and 90.9% specificity for a diagnosis of marginal zone lymphoma (MZL) while, CD148/180 > 5.15 was 100% specific and 81.8% sensitive for lymphoplasmacytic lymphoma. CD148^ab/T (>4.3; 100% specificity, 83.4% sensitivity) and CD148^ab/gr (>1.1; 100% sensitivity, 90% sensitivity) were useful for differentiating blastoid-mantle cell lymphoma/MCL from diffuse large B cell lymphoma; while CD148MFI (≥20.25), CD148^ab/T (>3.35) and CD148^ab/gr (>0.95) showed >90% specificity and sensitivity for distinguishing MCL from CLL. Pairwise analysis also showed a good discriminant function of various parameters for distinguishing SMZL from other MBNs like FL, MCL as well as CLL.

Conclusions: The current study shows an excellent utility of CD148MFI, CD180MFI, their ratio and relative expression levels in the subcategorization of immunophenotypically related MBNs.

Keywords: CD148; CD180; MYD88; flow cytometry; fluorescence in situ hybridization; mature B cell neoplasms; median fluorescence intensity.

MeSH terms

Adolescent
Adult
Aged
Aged, 80 and over
Antigens, CD / analysis*
B-Lymphocytes / pathology*
Bone Marrow / pathology*
Female
Flow Cytometry
Humans
Immunophenotyping
Leukemia, Lymphocytic, Chronic, B-Cell / diagnosis
Leukemia, Lymphocytic, Chronic, B-Cell / pathology
Lymphoma, B-Cell / diagnosis*
Lymphoma, B-Cell / pathology
Lymphoma, Mantle-Cell / diagnosis
Lymphoma, Mantle-Cell / pathology
Male
Middle Aged
Prospective Studies
Receptor-Like Protein Tyrosine Phosphatases, Class 3 / analysis
Young Adult

Substances

Antigens, CD
CD180 protein, human
PTPRJ protein, human
Receptor-Like Protein Tyrosine Phosphatases, Class 3