Abstract
The interaction of G protein-coupled receptors (GPCRs) with heterotrimeric G proteins plays a critical role in signal transduction processes, and multiple GPCR–G protein complexes reconstituted in detergent micelles have been visualized using cryo-EM. A new study reports the structure of neurotensin receptor 1 (NTSR1) in complex with the heterotrimeric Gi protein, assembled in a lipid environment using circularized nanodiscs. The structure sheds light on how the lipid context may influence receptor–G protein coupling and activation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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GTP-Binding Protein alpha Subunits, Gi-Go / chemistry
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GTP-Binding Protein alpha Subunits, Gi-Go / genetics
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GTP-Binding Protein alpha Subunits, Gi-Go / ultrastructure*
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Humans
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Lipids / chemistry*
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Multiprotein Complexes / chemistry
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Multiprotein Complexes / ultrastructure
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Protein Conformation*
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Receptors, Neurotensin / chemistry
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Receptors, Neurotensin / genetics
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Receptors, Neurotensin / ultrastructure*
Substances
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Lipids
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Multiprotein Complexes
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Receptors, Neurotensin
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neurotensin type 1 receptor
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GTP-Binding Protein alpha Subunits, Gi-Go