CircRNA circBACH1 (hsa_circ_0061395) serves as a miR-656-3p sponge to facilitate hepatocellular carcinoma progression through increasing SERBP1 expression

Biochem Biophys Res Commun. 2021 Jun 4:556:1-8. doi: 10.1016/j.bbrc.2021.03.136. Epub 2021 Apr 5.

Abstract

Hsa_circ_0061395(circBACH1) and SERBP1(SERPINE1 mRNA binding protein 1) have been reported to play a carcinogenic role in HCC.In this study, circBACH1, microRNA(miR)-656-3p, and SERBP1 expression levels with quantitative real-time polymerase chain reaction (qRT-PCR) in HCC tissue specimens and cells.The protein levels of SERBP1, E-Cadherin, vimentin, and N-Cadherin were detected with western blotting.Cell proliferation, migration, invasion, and apoptosis were determined with CCK-8, colony formation, transwell, and flow cytometry assays.The targeting relatio-nship between circBACH1 or SERBP1 and miR-656-3p was verified by dual-lucifer- ase reporter assay.The role of circBACH1 was validated by xenograft assay.CircBAC- H1 and SERBP1 were upregulated in HCC tissues and cells.Both circBACH1 and SERBP1 knockdown constrained proliferation, migration, invasion, and EMT(epithel-ial-mesenchymal transition), and facilitated apoptosis of HCC cells in vitro.Knockdo-wn of circBACH1 reduced HCC growth in vivo. SERBP1 overexpression partially neutralized the repressive effect of circBACH1 silencing on malignant behaviors of HCC cells.CircBACH1 sponged miR-656-3p to elevate SERBP1 expression, thereby accelerating the progression of HCC.The research provided a new evidence to support the role of circBACH1 in HCC.

Keywords: HCC; SERBP1; circBACH1; miR-656–3p.

MeSH terms

  • Animals
  • Carcinoma, Hepatocellular / genetics*
  • Carcinoma, Hepatocellular / pathology*
  • Cell Line, Tumor
  • Cell Proliferation
  • Disease Progression*
  • Female
  • Gene Expression Regulation, Neoplastic
  • Humans
  • In Vitro Techniques
  • Liver Neoplasms* / genetics
  • Liver Neoplasms* / pathology
  • Mice
  • Mice, Inbred BALB C
  • MicroRNAs / genetics*
  • RNA, Circular / deficiency
  • RNA, Circular / genetics*
  • RNA-Binding Proteins / antagonists & inhibitors
  • RNA-Binding Proteins / biosynthesis
  • RNA-Binding Proteins / genetics*
  • Xenograft Model Antitumor Assays

Substances

  • MIRN656 microRNA, human
  • MicroRNAs
  • RNA, Circular
  • RNA-Binding Proteins
  • SERBP1 protein, human