Comparison of CD34+ cells isolated from frozen cord blood and fresh adult peripheral blood of sickle cell disease patients in gene correction of the sickle mutation at late-stage erythroid differentiation

Comparison of CD34⁺ cells isolated from frozen cord blood and fresh adult peripheral blood of sickle cell disease patients in gene correction of the sickle mutation at late-stage erythroid differentiation

Br J Haematol. 2021 Sep;194(5):e80-e84. doi: 10.1111/bjh.17564. Epub 2021 Jun 1.

Chutima Kumkhaek¹, Naoya Uchida^{2

3}, John F Tisdale², Griffin P Rodgers¹

¹ Molecular and Clinical Hematology Branch, National Heart, Lung, and Blood Institute (NHLBI)/National Institute of Diabetes and Digestive and Kidney Disease (NIDDK), National Institutes of Health, Bethesda (NIH), Bethesda, MD, USA.
² Cellular and Molecular Therapeutics Branch, National Heart, Lung, and Blood Institute (NHLBI)/National Institute of Diabetes and Digestive and Kidney Disease (NIDDK), National Institutes of Health, Bethesda (NIH), Bethesda, MD, USA.
³ Division of Molecular and Medical Genetics, Center for Gene and Cell Therapy, The Institute of Medical Science, The University of Tokyo, Minato-ku, Tokyo, Japan.

No abstract available

Keywords: CD34+ cells; CRISPR/Cas9; cord blood; gene correction.; sickle mutation.