Association of GSTO1, GSTO2, GSTP1, GPX1 and SOD2 polymorphism with primary open angle glaucoma

Vesna Sobot; Miroslav Stamenkovic; Tatjana Simic; Djurdja Jerotic; Milica Djokic; Vesna Jaksic; Marija Bozic; Jovan Milic; Ana Savic-Radojevic; Tatjana Djukic

doi:10.1016/j.exer.2021.108863

Association of GSTO1, GSTO2, GSTP1, GPX1 and SOD2 polymorphism with primary open angle glaucoma

Exp Eye Res. 2022 Jan:214:108863. doi: 10.1016/j.exer.2021.108863. Epub 2021 Nov 24.

Authors

Affiliations

¹ Eye Clinic, University Clinical Center of Serbia, Pasterova 2, Belgrade, Serbia.
² University Eye Clinic, Medical Center Zvezdara, D. Tucovica 161, Belgrade, Serbia; Faculty of Special Education and Rehabilitation, University of Belgrade, Belgrade, Serbia.
³ Institute of Medical and Clinical Biochemistry, Pasterova 2, Belgrade, Serbia; Faculty of Medicine, University of Belgrade, Dr Subotica 8, Belgrade, Serbia; Serbian Academy of Sciences and Arts, Kneza Mihaila 35, Belgrade, Serbia.
⁴ Institute of Medical and Clinical Biochemistry, Pasterova 2, Belgrade, Serbia; Faculty of Medicine, University of Belgrade, Dr Subotica 8, Belgrade, Serbia.
⁵ Institute for Oncology and Radiology of Serbia, Pasterova 14, Belgrade, Serbia.
⁶ University Eye Clinic, Medical Center Zvezdara, D. Tucovica 161, Belgrade, Serbia; Faculty of Medicine, University of Belgrade, Dr Subotica 8, Belgrade, Serbia.
⁷ Eye Clinic, University Clinical Center of Serbia, Pasterova 2, Belgrade, Serbia; Faculty of Medicine, University of Belgrade, Dr Subotica 8, Belgrade, Serbia.
⁸ Eye Clinic, Clinical Center of Montenegro, Ljubljanska bb, Podgorica, Montenegro.
⁹ Institute of Medical and Clinical Biochemistry, Pasterova 2, Belgrade, Serbia; Faculty of Medicine, University of Belgrade, Dr Subotica 8, Belgrade, Serbia. Electronic address: tatjana.djukic@med.bg.ac.rs.

PMID: 34826418
DOI: 10.1016/j.exer.2021.108863

Abstract

It is becoming increasingly evident that oxidative stress has a supporting role in pathophysiology and progression of primary open angle glaucoma (POAG). The aim of our study was to assess the association between polymorphisms in genes encoding enzymes involved in redox homeostasis, mitochondrial superoxide dismutase (SOD2), glutathione peroxidase (GPX1) and glutathione transferases (GSTs) with susceptibility to POAG. Single nucleotide polymorphisms in GST omega (GSTO1rs4925, GSTO2 rs156697), pi 1 (GSTP1 rs1695), as well as GPX1 (rs1050450) and SOD2 (rs4880) were determined by quantitative polymerase chain reaction (qPCR) in 102 POAG patients and 302 respective controls. The risk for POAG development was noted in carriers of both GSTO2*GG and GSTO1*AA variant genotypes (OR = 8.21, p = 0.002). Individuals who carried GPX1*TT and SOD2*CC genotypes had also an increased risk of POAG development but without significance after Bonferroni multiple test correction (OR = 6.66, p = 0.005). The present study supports the hypothesis that in combination, GSTO1/GSTO2, modulate the risk of primary open angle glaucoma.

Keywords: Glutathione peroxidase 1; Glutathione transferases omega 1; Glutathione transferases omega 2; Glutathione transferases pi 1; Primary open angle glaucoma; Superoxide dismutase 2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Aged, 80 and over
Case-Control Studies
Female
Genetic Association Studies
Genetic Predisposition to Disease
Genotyping Techniques
Glaucoma, Open-Angle / diagnosis
Glaucoma, Open-Angle / genetics*
Glutathione Peroxidase / genetics*
Glutathione Peroxidase GPX1
Glutathione S-Transferase pi / genetics*
Glutathione Transferase / genetics*
Humans
Intraocular Pressure / physiology
Male
Middle Aged
Polymorphism, Single Nucleotide / genetics*
Real-Time Polymerase Chain Reaction
Superoxide Dismutase / genetics*

Substances

Glutathione Peroxidase
Superoxide Dismutase
superoxide dismutase 2
GSTO1 protein, human
GSTO2 protein, human
GSTP1 protein, human
Glutathione S-Transferase pi
Glutathione Transferase
Glutathione Peroxidase GPX1
GPX1 protein, human