Landscape of Cell Communication in Human Dental Pulp

Small Methods. 2021 Sep;5(9):e2100747. doi: 10.1002/smtd.202100747. Epub 2021 Aug 16.

Abstract

The cellular atlas of the stroma is not well understood. Here, the cell populations in human dental pulp through single-cell RNA sequencing are profiled. Dental pulp stem cells, pulp cells, T cells, macrophages, endothelial cells, and glial cells are identified in human dental pulp. These cells support each other through sending growth signals. Based on the appearance of ligand-receptor pairs between two cell populations, pulp cells have the greatest communication with other cell types, while T cells have the least communication. In addition, T cells expressing TLR1, TLR2, and TLR4, and endothelial cells expressing TLR4, monitor bacterial invasion. These findings provide the census of normal dental pulp.

Keywords: T cells; cell communication; dental pulp; macrophages; pulp cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Cell Differentiation
  • Dental Pulp / immunology*
  • Endothelial Cells / immunology
  • Exome Sequencing
  • Female
  • Gene Expression Profiling / methods*
  • Gene Expression Regulation
  • Humans
  • Sequence Analysis, RNA / methods
  • Single-Cell Analysis
  • T-Lymphocytes / immunology
  • Toll-Like Receptor 1 / genetics*
  • Toll-Like Receptor 2 / genetics*
  • Toll-Like Receptor 4 / genetics*

Substances

  • TLR1 protein, human
  • TLR2 protein, human
  • TLR4 protein, human
  • Toll-Like Receptor 1
  • Toll-Like Receptor 2
  • Toll-Like Receptor 4