Abstract
By genetic complementation in a yeast choline transport mutant from a yeast gene library, we isolated plasmids encoding choline transport. The cloned plasmids contained a common 4.0-kilobase DNA fragment and also complemented an ethanolamine transport defect. The cloned sequence present in the yeast genome was possibly unique.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Base Sequence
-
Biological Transport
-
Choline / metabolism*
-
Cloning, Molecular*
-
Ethanolamine
-
Ethanolamines / metabolism
-
Genes, Fungal*
-
Plasmids
-
Saccharomyces cerevisiae / genetics*
-
Saccharomyces cerevisiae / metabolism
Substances
-
Ethanolamines
-
Ethanolamine
-
Choline