Crystallization and preliminary X-ray investigation of uridine phosphorylase from Escherichia coli

J Biol Chem. 1987 Feb 25;262(6):2852-3.

Abstract

Crystals of uridine phosphorylase from Escherichia coli K12 have been grown from solutions of polyethylene glycol 4000. The crystals are trigonal, space group R3; the hexagonal axes are a = 154.4 A and c = 49.4 A. The crystals are quite stable to x-rays and diffract beyond 2.6 A resolution. It appears that the molecule is a hexamer with a subunit molecular weight of 27,500 and utilizes the 3-fold symmetry of the space group, resulting in two subunits/asymmetric unit.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Crystallization
  • Escherichia coli / enzymology*
  • Pentosyltransferases*
  • Uridine Phosphorylase*
  • X-Ray Diffraction

Substances

  • Pentosyltransferases
  • Uridine Phosphorylase