Problem: Cervical cancer has been recognized as the second most common cancer in women worldwide. Previous studies have reported that some circular RNAs (circRNAs) can influence the progression of cervical cancer. However, more researches are still required to unveil the underlying mechanism of how circRNAs regulate the progression of such cancer. We aimed at unveiling the mechanism of how circ_0060551 effected the progression of cervical cancer.
Method of study: RT-qPCR and western blot assays were used to detect the expression and protein levels. Mechanism experiments were conducted to investigate the relationship among circ_0060551, TPD52, miR-520a-5p and ELAVL1. Rescue assays were mainly carried out to verify how circ_0060551 influenced the migration and invasion of cervical cancer cells.
Results: According to the results, circ_0060551 was upregulated in cervical cancer cells and could promote the migration and invasion of cells via TPD52. In addition, circ_0060551 could up-regulate TPD52 expression through a ceRNA model to target miR-520a-5p. Moreover, circ_0060551 could stabilize the mRNA expression of TPD52 via recruiting ELAVL1.
Conclusion: Our study proved that circ_0060551 could promote the migration and invasion of cervical cancer cells.
Keywords: ELAVL1; TPD52; cervical cancer; circ_0060551; miR-520a-5p.
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