Objective To investigate the effect of miR-328-3p on the invasion and migration of colorectal cancer cells induced by interleukin-17 (IL-17) and its mechanism. Methods Real-time quantitative PCR was used to detect the expressions of miR-328-3p, FOXP3, and IL-17 mRNA in FHC colorectal epithelial cells and colorectal cancer cells (HCT116, Caco-2, and LoVo). TranswellTM assay was used to detect cell invasion and migration, and gelatin zymography assay to detect the activities of matrix metalloproteinases 2 (MMP2) and MMP9. Dual luciferase reporter assay was conducted to detect the targeted regulation relationship between miR-328-3p and FOXP3. The level of FOXP3 protein in Caco-2 cells was detected by Western blot analysis. Results Compared with those in colorectal epithelial cells, the expression level of miR-328-3p in colorectal cancer cells decreased, while the levels of FOXP3 and IL-17 increased. Transfection of miR-328-3p mimics inhibited the invasion and migration ability of IL-17-induced Caco-2 cells and the activities of MMP2 and MMP9 in the cells. miR-328-3p directly targeted and inhibited the protein level of FOXP3 in Caco-2 cells. Overexpression of FOXP3 partly reversed the effect of miR-328-3p mimics on the invasion and migration ability of IL-17-induced Caco-2 cells and the activities of MMP2 and MMP9 in the cells. Conclusion miR-328-3p inhibits the invasion and migration of IL-17-induced Caco-2 cells by down-regulating FOXP3.