Hsa_circ_0008673 Promotes Breast Cancer Progression by MiR-578/GINS4 Axis

Background: Circular RNAs (circRNAs) play a crucial role in breast cancer (BC) development. This study aimed to explore the new potential mechanism of hsa_circ_0008673 in BC.

Materials and methods: Hsa_circ_0008673, microRNA-578 (miR-578) and recombinant human GINS complex subunit 4 (GINS4) abundances were measured via quantitative real-time PCR or western blot. Cell proliferation, metastasis, angiogenesis and apoptosis were assessed via EdU assay, transwell assay, tube formation assay, and flow cytometry. The interactions among hsa_circ_0008673, miR-578 and GINS4 were tested via dual-luciferase reporter analysis and RNA pull-down assay. Animal studies were performed to assess the effect of hsa_circ_0008673 on BC tumor growth.

Results: Hsa_circ_0008673 level was increased in BC tissues and cells. Hsa_circ_0008673 silencing repressed BC cell growth, metastasis and angiogenesis, as well as hampered BC tumor growth. Hsa_circ_0008673 acted as miR-578 sponge, and miR-578 targeted GINS4. Furthermore, hsa_circ_0008673 modulated GINS4 expression through sponging miR-578. Additionally, miR-578 inhibitor or GINS4 overexpression could reverse the inhibitory effect of hsa_circ_0008673 silencing on BC cell progression.

Conclusion: Hsa_circ_0008673 might promote BC progression via modulating miR-578/GINS4 pathway.