FOXO3a functions as a transcriptional and co-transcriptional splicing regulator in vascular endothelial cell lines

Gene. 2024 Apr 30:904:148221. doi: 10.1016/j.gene.2024.148221. Epub 2024 Jan 28.

Abstract

Recent studies have indicated a connection between Forkhead box O3a protein and coronary artery disease, yet the exact role of FOXO3a in the regulation of metabolic processes and apoptosis in vascular endothelial cells is still unknown. Therefore, we investigated the role of FOXO3a on target genes in a human vascular endothelial cell line. Through the utilization of high-throughput sequencing technology, we analyzed gene expression profiles and alternative splicing patterns in human vascular endothelial cells with FOXO3a over expression. This study identified 419 DEGs between FOXO3a-OE HUVEC model and control cells. KEGG analysis indicated that the upregulated genes were mainly enriched in inflammation-related signaling pathways, and the downregulated genes were enriched in lipid metabolism-related pathways.

Keywords: Alternative splicing; Coronary heart disease; FOXO3a; Targeted regulation.

MeSH terms

  • Apoptosis / genetics
  • Endothelial Cells* / metabolism
  • Forkhead Box Protein O3 / genetics
  • Forkhead Box Protein O3 / metabolism
  • Forkhead Transcription Factors* / genetics
  • Forkhead Transcription Factors* / metabolism
  • Humans
  • Signal Transduction / genetics

Substances

  • Forkhead Box Protein O3
  • Forkhead Transcription Factors
  • FOXO3 protein, human