Abstract
Two mannose-negative mutants of Escherichia coli K-12 have been isolated. These mutants are deficient in the ability to synthesize phosphomannose isomerase and capsular polysaccharide when grown on glucose-containing media. Interrupted mating experiments to determine the kinetics of genetic transfer show that the two mannose-negative mutations map together between the histidine and tryptophan regions of the E. coli chromosome.
MeSH terms
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Chromosome Mapping
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Chromosomes, Bacterial
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Conjugation, Genetic
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Escherichia coli / enzymology*
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Escherichia coli / growth & development
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Genetics, Microbial*
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Glucose / metabolism
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Glucose-6-Phosphate Isomerase / metabolism
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Glucosephosphate Dehydrogenase / metabolism
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Histidine
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Isomerases / biosynthesis
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Isomerases / metabolism*
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Mannose / metabolism
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Mutation
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Polysaccharides, Bacterial / biosynthesis
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Recombination, Genetic
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Streptomycin / pharmacology
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Tryptophan
Substances
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Polysaccharides, Bacterial
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Histidine
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Tryptophan
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Glucosephosphate Dehydrogenase
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Isomerases
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Glucose-6-Phosphate Isomerase
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Glucose
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Mannose
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Streptomycin