Abstract
sds22 is a regulatory polypeptide of protein phosphatase-1 that is required for the completion of mitosis in both fission and budding yeast. We report here the cDNA cloning of a human polypeptide that is 46% identical to yeast sds22. The human homolog of sds22 consists of 360 residues, has a calculated molecular mass of 41.6 kDa and shows a tandem array of 11 leucine-rich repeat structures of 22 residues. Northern analysis revealed a major transcript of 1.39 kb in all 8 investigated human tissues. sds22 was detected by western analysis in both the cytoplasm and the nucleus of rat liver cells as a polypeptide of 44 kDa.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Blotting, Western
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Cell Cycle Proteins / analysis
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Cell Cycle Proteins / biosynthesis*
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Cell Cycle Proteins / chemistry
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Cell Nucleus / metabolism*
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Consensus Sequence
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Cytosol / metabolism
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DNA Primers
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Female
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Fungal Proteins / analysis
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Fungal Proteins / biosynthesis*
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Fungal Proteins / chemistry
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Humans
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Liver / metabolism*
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Molecular Sequence Data
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Molecular Weight
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Nuclear Proteins*
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Organ Specificity
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Phosphoprotein Phosphatases / metabolism*
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Polymerase Chain Reaction
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Pregnancy
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Protein Phosphatase 1
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Rats
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Schizosaccharomyces / genetics
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Schizosaccharomyces / metabolism
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Schizosaccharomyces pombe Proteins*
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Sequence Homology, Amino Acid
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Transcription, Genetic
Substances
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Cell Cycle Proteins
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DNA Primers
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Fungal Proteins
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Nuclear Proteins
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PPP1R7 protein, human
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Ppp1r7 protein, rat
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Schizosaccharomyces pombe Proteins
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sds22 protein, S pombe
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Phosphoprotein Phosphatases
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Protein Phosphatase 1