Abstract
Previously, we studied the surface expression of LFA-1 (CD11a/CD18) and ICAM-1 (CD54) in a panel of 16 human T-cell lines and found that those carrying HTLV-1 pro-viruses expressed high levels of ICAM-1. Enhanced expression of ICAM-1 was also seen in fresh leukemic cells from ATL patients. In the present study, we systematically examined the surface expression of 19 different cell-adhesion molecules (CAMs) (the immunoglobulin superfamily, the homing receptors, the integrins, etc.) in a panel of 20 human T-cell lines (6 HTLV-1-negative lines and 14HTLV-1-positive lines). The surface expression of LFA-3 (CD58) was constantly enhanced in HTLV-1-positive T-cell lines except for MT-1, an ATL-derived cell line, which was devoid of a number of CAMs including ICAM-1. LFA-3 proteins and mRNA were found at strongly increased levels in HTLV-1-positive T-cell lines.
MeSH terms
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Antigens, CD / analysis*
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Antigens, CD / genetics
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Antigens, Differentiation, T-Lymphocyte / analysis
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Blotting, Northern
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CD2 Antigens
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CD28 Antigens / analysis
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CD58 Antigens
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Cell Adhesion Molecules / analysis*
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Flow Cytometry
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Fluorescent Antibody Technique
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Gene Expression
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Human T-lymphotropic virus 1 / immunology*
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Humans
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Hyaluronan Receptors
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Immunoblotting
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Integrins / analysis
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Leukemia, T-Cell / immunology*
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Leukosialin
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Membrane Glycoproteins / analysis*
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Membrane Glycoproteins / genetics
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RNA, Messenger / analysis
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Receptors, Immunologic / analysis
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Receptors, Lymphocyte Homing / analysis
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Sialoglycoproteins / analysis
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T-Lymphocytes / immunology*
Substances
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Antigens, CD
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Antigens, Differentiation, T-Lymphocyte
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CD2 Antigens
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CD28 Antigens
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CD58 Antigens
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Cell Adhesion Molecules
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Hyaluronan Receptors
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Integrins
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Leukosialin
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Membrane Glycoproteins
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RNA, Messenger
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Receptors, Immunologic
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Receptors, Lymphocyte Homing
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SPN protein, human
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Sialoglycoproteins