Tyrosine phosphorylation of the proto-oncogene product Vav and its association with the adapter Grb2/Ash in a human leukemia cell line UT-7

Jpn J Cancer Res. 1995 Apr;86(4):336-41. doi: 10.1111/j.1349-7006.1995.tb03061.x.

Abstract

The vav proto-oncogene product (Vav) is expressed exclusively in hematopoietic cells and is reported to have guanine nucleotide exchange activity. Here we report that granulocyte-macrophage colony-stimulating factor, interleukin-3, and erythropoietin induce tyrosine phosphorylation of Vav in a human leukemia cell line UT-7. Tyrosine phosphorylation of Vav is rapid and transient; it occurs within 1 min of the stimulation and at physiological concentrations of the factors. Furthermore, we show that Vav is constitutively associated with the adapter molecule Grb2/Ash in UT-7. These data suggest that tyrosine kinases, the adapter Grb2/Ash, and the guanine nucleotide exchange factor Vav are members of a signaling pathway leading to Ras activation in hematopoietic cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing*
  • Amino Acid Sequence
  • Cell Cycle Proteins*
  • ErbB Receptors / metabolism
  • Erythropoietin / pharmacology
  • GRB2 Adaptor Protein
  • Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
  • Humans
  • Interleukin-3 / pharmacology
  • Leukemia / genetics
  • Leukemia / metabolism*
  • Molecular Sequence Data
  • Neoplasm Proteins / metabolism*
  • Phosphorylation / drug effects
  • Protein-Tyrosine Kinases / metabolism*
  • Proteins / metabolism*
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-vav
  • Proto-Oncogene Proteins pp60(c-src) / metabolism
  • Signal Transduction / physiology
  • Stimulation, Chemical
  • Tumor Cells, Cultured
  • ras Proteins / metabolism

Substances

  • Adaptor Proteins, Signal Transducing
  • Cell Cycle Proteins
  • GRB2 Adaptor Protein
  • GRB2 protein, human
  • Interleukin-3
  • MAS1 protein, human
  • Neoplasm Proteins
  • Proteins
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-vav
  • VAV1 protein, human
  • Erythropoietin
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • ErbB Receptors
  • Protein-Tyrosine Kinases
  • Proto-Oncogene Proteins pp60(c-src)
  • ras Proteins