Abstract
A polymerase chain reaction assay (Lust J.A. et al. (1992). Cytokine 4:96-100) was used to detect a mRNA coding for a soluble IL-6 receptor in human hepatoma cells. In addition to the expected amplification product, we found a sequence (SR4) which could be aligned to it with 78% identity. After cloning and sequencing a genomic 2.5-2.7 kB EcoRI fragment containing SR4, this sequence turned out to be part of a pseudogene corresponding to the transmembrane domain deleted soluble IL-6 receptor. Screening of a panel of interspecies hybrids revealed that it maps to chromosome 9.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, CD / genetics*
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Base Sequence
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Blotting, Southern
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Carcinoma, Hepatocellular / metabolism
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Chromosome Mapping
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Chromosomes, Human, Pair 9
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Cloning, Molecular
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DNA Primers
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Genomic Library
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Humans
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Molecular Sequence Data
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Polymerase Chain Reaction
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Pseudogenes*
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RNA, Messenger / chemistry
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RNA, Messenger / isolation & purification*
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Receptors, Interleukin / genetics*
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Receptors, Interleukin-6
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Sequence Alignment
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Tumor Cells, Cultured
Substances
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Antigens, CD
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DNA Primers
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RNA, Messenger
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Receptors, Interleukin
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Receptors, Interleukin-6