Abstract
DNA-activated protein kinase (DNA-PK) is a serine/threonine protein kinase that interacts with a DNA end-binding heterodimeric protein, Ku, and is activated by double-stranded DNA. Genomic clones that contain the DNA-PK gene complement the murine scid defect, indicating that DNA-PK affects double-strand break repair and V(D)J recombination. Here we describe the cDNA sequence of the region that corresponds to about 100 kDa of C-terminal sequence of this large (> p350 kDa) protein. This region contains a kinase domain that has strong homology to phosphatidylinositol kinases.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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1-Phosphatidylinositol 4-Kinase
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Amino Acid Sequence
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Base Sequence
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DNA, Complementary / genetics*
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DNA-Activated Protein Kinase
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DNA-Binding Proteins*
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Humans
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Molecular Sequence Data
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Nuclear Proteins
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Phosphotransferases (Alcohol Group Acceptor) / genetics*
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Protein Serine-Threonine Kinases / genetics*
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Sequence Analysis
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Sequence Homology, Amino Acid
Substances
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DNA, Complementary
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DNA-Binding Proteins
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Nuclear Proteins
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Phosphotransferases (Alcohol Group Acceptor)
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1-Phosphatidylinositol 4-Kinase
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DNA-Activated Protein Kinase
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PRKDC protein, human
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Protein Serine-Threonine Kinases