bcl-1, bcl-2, p53, c-myc, and lyt-10 analysis in cutaneous lymphomas

Recent Results Cancer Res. 1995:139:249-61. doi: 10.1007/978-3-642-78771-3_19.

Abstract

In the present study we investigated the pathogenetic role of c-myc, bcl-2, and lyt-10 oncogenes, bcl-1 locus, and p53 suppressor gene in a representative panel of cutaneous lymphomas, including 25 cases of cutaneous B cell lymphoma (CBCL) and 29 cases of cutaneous T cell lymphoma (CTCL). In our analysis four cases of CBCL were found rearranged for bcl-2 and two for the bcl-1 locus. Two cases of CTCL and one case of CBCL were found rearranged for lyt-10. No rearrangements of c-myc oncogene were found in CBCL. Analysis of p53 gene showed mutation only in one case of mycosis fungoides in tumoral stage, at codon 163 of p53 gene (TAC-->CAC; Tyr--> Asp). Our data suggest that in primary CBCL bcl-2 oncogenes and bcl-1 locus are rarely involved. Furthermore, in primary CTCL p53 gene is not affected at significant frequency. The occurrence of p53 mutation in a patient affected by mycosis fungoides in tumoral stage may represent an involvement of p53 gene in tumor progression of CTCL, a finding observed in several types of human cancer.

MeSH terms

  • Base Sequence
  • Chromosome Aberrations
  • Cyclin D1
  • DNA Mutational Analysis
  • DNA, Neoplasm / genetics*
  • Gene Rearrangement, B-Lymphocyte, Heavy Chain
  • Gene Rearrangement, T-Lymphocyte
  • Genes, Immunoglobulin
  • Genes, myc
  • Genes, p53*
  • Humans
  • Lymphoma, B-Cell / classification
  • Lymphoma, B-Cell / genetics*
  • Lymphoma, B-Cell / pathology
  • Lymphoma, T-Cell, Cutaneous / classification
  • Lymphoma, T-Cell, Cutaneous / genetics*
  • Lymphoma, T-Cell, Cutaneous / pathology
  • Molecular Sequence Data
  • Mycosis Fungoides / genetics
  • NF-kappa B / genetics
  • NF-kappa B p52 Subunit
  • Oncogenes*
  • Polymerase Chain Reaction
  • Polymorphism, Single-Stranded Conformational
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins c-bcl-2
  • Receptors, Antigen, T-Cell, alpha-beta / genetics
  • Receptors, Antigen, T-Cell, gamma-delta / genetics
  • Skin Neoplasms / genetics*
  • Skin Neoplasms / pathology

Substances

  • DNA, Neoplasm
  • NF-kappa B
  • NF-kappa B p52 Subunit
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • Receptors, Antigen, T-Cell, alpha-beta
  • Receptors, Antigen, T-Cell, gamma-delta
  • Cyclin D1