Transcriptional regulation of carbonic anhydrase II by retinoic acid in the human pancreatic tumor cell line DANG

FEBS Lett. 1995 Jul 10;368(1):45-8. doi: 10.1016/0014-5793(95)00596-2.

Abstract

Carbonic anhydrase II (CA II) generates bicarbonate in human pancreatic duct cells. We have developed the human pancreatic duct cell line DANG as a model to study the effects of all-trans-retinoic acid (ATRA) on CA II gene expression. ATRA treatment resulted in a time- and dose-dependent inhibition of CA II mRNA concentrations in DANG cells. These inhibitory effects were paralleled by a time-dependent decrease of CA II protein concentrations. Nuclear run on analysis revealed that the decrease of CA II mRNA concentrations was due to a decreased rate of CA II gene transcription. These data show that ATRA transcriptionally modulates CA II gene expression in human pancreatic carcinoma cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbonic Anhydrases / genetics*
  • Gene Expression Regulation, Enzymologic* / drug effects
  • Gene Expression Regulation, Neoplastic* / drug effects
  • Humans
  • Pancreas / cytology
  • Pancreas / metabolism*
  • Pancreatic Neoplasms / drug therapy
  • Pancreatic Neoplasms / enzymology*
  • Pancreatic Neoplasms / genetics
  • Transcription, Genetic / drug effects
  • Tretinoin / pharmacology*
  • Tumor Cells, Cultured

Substances

  • Tretinoin
  • Carbonic Anhydrases