Abstract
Ciliary neurotrophic factor (CNTF) has been described as a neuro-active cytokine that shares functional similarities with the leukemia inhibitory factor (LIF). We demonstrate here that, like LIF, CNTF stimulates expression of acute phase plasma proteins in rat H-35 hepatoma cells. Transfection of the LIF receptor into Hep3B hepatoma cells reconstituted LIF and oncostatin M regulation of acute phase plasma protein genes. Co-expression of the LIF receptor and the CNTF receptor, but not expression of either subunit alone, generated CNTF responsiveness in Hep3B cells, suggesting cooperativity of these receptor subunits. Evidence is presented for direct interaction of the LIF receptor with the intracellular signal transduction machinery.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Acute-Phase Proteins / metabolism
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Animals
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Carcinoma, Hepatocellular
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Ciliary Neurotrophic Factor
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Growth Inhibitors / metabolism*
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Humans
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Interleukin-6*
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Leukemia Inhibitory Factor
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Leukemia Inhibitory Factor Receptor alpha Subunit
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Liver / metabolism
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Lymphokines / metabolism*
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Mice
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Nerve Tissue Proteins / metabolism*
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Oncostatin M
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Peptides / metabolism*
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Rats
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Receptor, Ciliary Neurotrophic Factor
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / metabolism
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Receptors, Cytokine*
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Receptors, Immunologic / genetics
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Receptors, Immunologic / metabolism
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Receptors, OSM-LIF
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Transfection
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Tumor Cells, Cultured
Substances
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Acute-Phase Proteins
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Ciliary Neurotrophic Factor
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Growth Inhibitors
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Interleukin-6
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LIF protein, human
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LIFR protein, human
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Leukemia Inhibitory Factor
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Leukemia Inhibitory Factor Receptor alpha Subunit
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Lif protein, mouse
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Lifr protein, mouse
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Lifr protein, rat
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Lymphokines
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Nerve Tissue Proteins
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OSM protein, human
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Osm protein, mouse
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Peptides
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Receptor, Ciliary Neurotrophic Factor
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Receptors, Cell Surface
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Receptors, Cytokine
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Receptors, Immunologic
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Receptors, OSM-LIF
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Oncostatin M