Abstract
A patient with glioblastoma multiforme (GBM) who had failed conventional therapy was treated with IL-2 gene therapy. The patient received 10 subcutaneous immunizations with autologous tumor cells and fibroblasts genetically modified to secrete IL-2 by retroviral gene transfer. An antitumor immune response mediated in part by CD8+ cytotoxic T cells was demonstrated with the patient's peripheral blood mononuclear cells. A magnetic resonance imaging (MRI) scan performed 4 weeks after the highest treatment dose revealed marked tumor necrosis. These results support the evaluation of this form of IL-2 gene therapy in additional patients with glioblastoma.
Publication types
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Case Reports
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antineoplastic Combined Chemotherapy Protocols / therapeutic use
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Brain Neoplasms / immunology
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Brain Neoplasms / pathology
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Brain Neoplasms / therapy*
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Cells, Cultured / transplantation
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Combined Modality Therapy
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Fatal Outcome
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Female
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Fibroblasts / metabolism*
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Fibroblasts / transplantation*
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Genetic Therapy*
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Genetic Vectors
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Glioblastoma / immunology
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Glioblastoma / pathology
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Glioblastoma / therapy*
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Humans
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Injections, Subcutaneous
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Interleukin-2 / genetics
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Interleukin-2 / metabolism
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Interleukin-2 / therapeutic use*
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Isotretinoin / therapeutic use
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Lomustine / administration & dosage
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Magnetic Resonance Imaging
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Middle Aged
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Necrosis
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Neoplasm Recurrence, Local
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Neoplastic Stem Cells / metabolism*
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Neoplastic Stem Cells / transplantation*
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Procarbazine / administration & dosage
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Radioimmunotherapy
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Radiosurgery
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Recombinant Fusion Proteins / metabolism
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Recombinant Fusion Proteins / therapeutic use*
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Retroviridae / genetics
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Salvage Therapy*
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T-Lymphocytes, Cytotoxic / immunology
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Tamoxifen / therapeutic use
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Temporal Lobe*
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Transfection
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Tumor Cells, Cultured / transplantation
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Vincristine / administration & dosage
Substances
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Interleukin-2
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Recombinant Fusion Proteins
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Tamoxifen
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Procarbazine
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Vincristine
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Lomustine
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Isotretinoin