Interleukin-2 gene therapy in a patient with glioblastoma

Gene Ther. 1995 Mar;2(2):164-7.

Abstract

A patient with glioblastoma multiforme (GBM) who had failed conventional therapy was treated with IL-2 gene therapy. The patient received 10 subcutaneous immunizations with autologous tumor cells and fibroblasts genetically modified to secrete IL-2 by retroviral gene transfer. An antitumor immune response mediated in part by CD8+ cytotoxic T cells was demonstrated with the patient's peripheral blood mononuclear cells. A magnetic resonance imaging (MRI) scan performed 4 weeks after the highest treatment dose revealed marked tumor necrosis. These results support the evaluation of this form of IL-2 gene therapy in additional patients with glioblastoma.

Publication types

  • Case Reports
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antineoplastic Combined Chemotherapy Protocols / therapeutic use
  • Brain Neoplasms / immunology
  • Brain Neoplasms / pathology
  • Brain Neoplasms / therapy*
  • Cells, Cultured / transplantation
  • Combined Modality Therapy
  • Fatal Outcome
  • Female
  • Fibroblasts / metabolism*
  • Fibroblasts / transplantation*
  • Genetic Therapy*
  • Genetic Vectors
  • Glioblastoma / immunology
  • Glioblastoma / pathology
  • Glioblastoma / therapy*
  • Humans
  • Injections, Subcutaneous
  • Interleukin-2 / genetics
  • Interleukin-2 / metabolism
  • Interleukin-2 / therapeutic use*
  • Isotretinoin / therapeutic use
  • Lomustine / administration & dosage
  • Magnetic Resonance Imaging
  • Middle Aged
  • Necrosis
  • Neoplasm Recurrence, Local
  • Neoplastic Stem Cells / metabolism*
  • Neoplastic Stem Cells / transplantation*
  • Procarbazine / administration & dosage
  • Radioimmunotherapy
  • Radiosurgery
  • Recombinant Fusion Proteins / metabolism
  • Recombinant Fusion Proteins / therapeutic use*
  • Retroviridae / genetics
  • Salvage Therapy*
  • T-Lymphocytes, Cytotoxic / immunology
  • Tamoxifen / therapeutic use
  • Temporal Lobe*
  • Transfection
  • Tumor Cells, Cultured / transplantation
  • Vincristine / administration & dosage

Substances

  • Interleukin-2
  • Recombinant Fusion Proteins
  • Tamoxifen
  • Procarbazine
  • Vincristine
  • Lomustine
  • Isotretinoin