Abstract
A human signet ring gastric carcinoma cell line TSGH9201 was established in vitro. The cells grew in vitro as a monolayer with polygonal morphology and had a population doubling time of 34 hours. The cells secreted tumor markers CEA and CA 125. They were, however, not tumorigenic in athymic nude mice. Karyotypic analysis demonstrated a near tetraploidy with a modal chromosome number of 98. Northern blotting and immunocytochemical analysis revealed the expression of both transforming growth factor alpha and high levels of epidermal growth factor receptor. Cell growth was inhibited by the epidermal growth factor in vitro. The cell line may be a useful tool to study autocrine growth regulation through the epidermal growth factor receptor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Northern
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Carcinoma, Signet Ring Cell / drug therapy*
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Carcinoma, Signet Ring Cell / metabolism
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Carcinoma, Signet Ring Cell / pathology
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Cell Division / drug effects
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DNA, Neoplasm / genetics
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Epidermal Growth Factor / pharmacology*
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ErbB Receptors / analysis
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ErbB Receptors / drug effects*
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ErbB Receptors / genetics
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Gene Expression Regulation, Neoplastic
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Humans
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Mice
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Mice, Nude
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Polyploidy
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RNA, Neoplasm / isolation & purification
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Stomach Neoplasms / drug therapy*
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Stomach Neoplasms / metabolism
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Stomach Neoplasms / pathology
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Transforming Growth Factor alpha / analysis
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Transforming Growth Factor alpha / drug effects*
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Transforming Growth Factor alpha / genetics
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Tumor Cells, Cultured / cytology
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Tumor Cells, Cultured / drug effects*
Substances
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DNA, Neoplasm
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RNA, Neoplasm
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Transforming Growth Factor alpha
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Epidermal Growth Factor
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ErbB Receptors