Elements in the long terminal repeat of HIV-1 that interact with nuclear extracts from Jurkat cells persistently infected with vaccinia virus

P Y Chang; K Stellrecht; S Melana; B G Pogo

doi:10.1016/0168-1702(94)90095-7

Elements in the long terminal repeat of HIV-1 that interact with nuclear extracts from Jurkat cells persistently infected with vaccinia virus

Virus Res. 1994 Nov;34(2):127-38. doi: 10.1016/0168-1702(94)90095-7.

Authors

P Y Chang¹, K Stellrecht, S Melana, B G Pogo

Affiliation

¹ Department of Microbiology, Mount Sinai School of Medicine, New York, NY 10029.

PMID: 7856307
DOI: 10.1016/0168-1702(94)90095-7

Abstract

Previous reports showed transactivation of the long terminal repeat (LTR) of HIV-1 in Jurkat cells persistently infected with vaccinia virus. In this communication, electrophoretic mobility shift assays were used to characterize the elements in HIV-1 LTR which might be responsible for the mechanism of transactivation. The results indicated that two elements, those for binding NF-kB and NFAT-1, were able to interact with nuclear extracts derived from Jurkat cells persistently infected with vaccinia virus, suggesting that they may play a role in the transactivation of HIV-1 LTR.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Binding Sites
Binding, Competitive
Cell Line
Cell Nucleus / metabolism*
Cell Transformation, Viral
DNA, Viral / metabolism
DNA-Binding Proteins / metabolism
Enhancer Elements, Genetic
HIV Long Terminal Repeat*
HIV-1 / genetics*
Humans
Molecular Sequence Data
NF-kappa B / metabolism
NFATC Transcription Factors
Nuclear Proteins / metabolism*
Oligodeoxyribonucleotides
Restriction Mapping
Substrate Specificity
Transcription Factors / metabolism
Transcriptional Activation*
Vaccinia virus / genetics*

Substances

DNA, Viral
DNA-Binding Proteins
NF-kappa B
NFATC Transcription Factors
Nuclear Proteins
Oligodeoxyribonucleotides
Transcription Factors