Abstract
Two new human mammary carcinoma lines originating from surgical material were established in nude mice. According to the adopted criteria, the tumor 4049 has been classified as estradiol receptor positive and mammary carcinoma 4296 as estradiol receptor negative. Both tumors proved to be c-erbB-2 protein positive and EGF-receptor negative. In contrast to carcinoma 4296, the in vitro growth and the take rate of mammary carcinoma 4049 in nude mice seems to be dependent on stromal components. Pretreatment of mice with estradiol/peanut oil before tumor engraftment was an essential precondition for the growth of the primary tumor in nude mice. After successful establishment the tumor growth was significantly stimulated by estradiol. The growth rate of mammary carcinoma 4296 was independent of any supplementation of estradiol. The two breast tumors were characterized with regard to their growth behaviour, histology, and sensitivity to cytostatics and antihormones. They are considered suitable tumor models for the testing of antineoplastic substances and for biological experiments.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents / therapeutic use
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Breast Neoplasms / drug therapy
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Breast Neoplasms / metabolism
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Breast Neoplasms / pathology*
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Carcinoma, Ductal, Breast / drug therapy
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Carcinoma, Ductal, Breast / metabolism
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Carcinoma, Ductal, Breast / pathology*
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Carcinoma, Squamous Cell / drug therapy
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Carcinoma, Squamous Cell / metabolism
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Carcinoma, Squamous Cell / pathology*
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Cell Division
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Connective Tissue / physiology*
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ErbB Receptors / analysis
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Estradiol* / administration & dosage
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Estradiol* / pharmacology
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Female
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Gene Expression Regulation, Neoplastic / drug effects
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Graft Survival / drug effects
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Humans
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Karyotyping
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Male
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Mice
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Mice, Nude
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Neoplasm Proteins / analysis*
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Neoplasm Transplantation
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Neoplasms, Hormone-Dependent / drug therapy
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Neoplasms, Hormone-Dependent / metabolism
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Neoplasms, Hormone-Dependent / pathology
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Peanut Oil
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Plant Oils / pharmacology
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Postmenopause
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Premenopause
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Receptor, ErbB-2 / analysis
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Receptor, ErbB-2 / biosynthesis
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Receptor, ErbB-2 / genetics
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Receptors, Estradiol / analysis*
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Receptors, Progesterone / analysis
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Receptors, Progesterone / biosynthesis
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Receptors, Progesterone / genetics
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Tamoxifen / pharmacology
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Transplantation, Heterologous
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Tumor Cells, Cultured*
Substances
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Antineoplastic Agents
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Neoplasm Proteins
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Peanut Oil
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Plant Oils
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Receptors, Estradiol
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Receptors, Progesterone
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Tamoxifen
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Estradiol
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ErbB Receptors
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Receptor, ErbB-2