Abstract
The human iron-binding protein melanotransferrin is up-regulated in most skin melanomas. With the goal to understand the mechanisms controlling the expression of the gene in these tumor cells, we previously reported the identification of an enhancer exhibiting melanoma specificity. We show here that, in the highly expressing SK-MEL-28 melanoma cell line, the chromatin structure in the enhancer region is in an open configuration and that the transcription factors governing its activity belong to the helix-loop-helix and the Jun/Fos leucine zipper families.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Neoplasm / genetics*
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Antigens, Neoplasm / metabolism
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Base Sequence
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Binding Sites
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DNA, Neoplasm
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Deoxyribonuclease I / metabolism
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Enhancer Elements, Genetic*
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Gene Expression Regulation, Neoplastic*
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HeLa Cells
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Helix-Loop-Helix Motifs
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Humans
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Melanoma / genetics*
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Melanoma-Specific Antigens
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Molecular Sequence Data
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Neoplasm Proteins / genetics*
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Neoplasm Proteins / metabolism
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Transcription Factors / metabolism
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Transcriptional Activation
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Tumor Cells, Cultured
Substances
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Antigens, Neoplasm
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DNA, Neoplasm
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Melanoma-Specific Antigens
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Neoplasm Proteins
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Transcription Factors
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Deoxyribonuclease I