Abstract
In this report we analyzed the mRNA expression of the resistance-related enzymes DNA topoisomerase II (Topo II), thymidylate synthase (TS), glutathione S-transferase-pi (GST-pi) and glutathione peroxidase (GP) in childhood acute lymphoblastic leukemia (ALL) and their correlation to the proliferative activity, determined by Ki-67. RNA of blast cells from 54 children with untreated ALL were examined by dot blot hybridization. We found a significant positive correlation between Topo II and TS and cell proliferation. No significant correlation was detected between the mRNA expression of the glutathione-dependent enzymes GST-pi or GP and Ki-67. The results were substantiated by a semiquantitative RT-PCR-assay and by immunocytochemistry.
MeSH terms
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Cell Division
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Child
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Child, Preschool
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DNA Topoisomerases, Type II / analysis
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DNA Topoisomerases, Type II / biosynthesis
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DNA Topoisomerases, Type II / genetics*
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Female
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Gene Expression
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Glutathione Peroxidase / analysis
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Glutathione Peroxidase / biosynthesis
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Glutathione Peroxidase / genetics*
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Glutathione Transferase / analysis
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Glutathione Transferase / biosynthesis
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Glutathione Transferase / genetics*
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Humans
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Immunoblotting
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Immunohistochemistry
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Infant
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Ki-67 Antigen
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Male
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Neoplasm Proteins / analysis
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Neoplasm Proteins / biosynthesis
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Neoplasm Proteins / genetics*
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Nuclear Proteins / genetics
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Polymerase Chain Reaction
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Precursor Cell Lymphoblastic Leukemia-Lymphoma / enzymology*
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Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics*
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Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology
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RNA, Messenger / genetics
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Thymidylate Synthase / analysis
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Thymidylate Synthase / biosynthesis
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Thymidylate Synthase / genetics*
Substances
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Ki-67 Antigen
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Neoplasm Proteins
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Nuclear Proteins
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RNA, Messenger
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Glutathione Peroxidase
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Thymidylate Synthase
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Glutathione Transferase
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DNA Topoisomerases, Type II