Abstract
Molecular analysis of the t(10;14) chromosomal translocation found in pediatric patients with T-cell acute lymphoblastic leukemia has led to the identification of the HOX-11 (TCL-3) protooncogene. The HOX-11 cDNA contains an open reading frame encoding a homeoprotein with features of DNA-binding. The majority of the t(10;14) chromosomal translocation breakpoints have been mapped to the 5' end of the HOX-11 gene, supporting the notion that deregulation of the HOX-11 gene by the t(10;14) chromosomal translocation contributed importantly to leukemia formation. To further define the role of the HOX-11 homeoprotein, we have prepared rabbit antiserum against a trpE-HOX-11 fusion protein. The purified anti-HOX-11 IgG immuno-precipitated a protein with apparent relative molecular mass of 40 kD. Biochemical fractionation demonstrated that the protein is localized in the nucleus. Furthermore, the HOX-11 RNA and protein appeared to be modulated during the cell cycle, with the highest level of expression at G1/S phase boundary. Taken together, these data suggest that the HOX-11 gene product may function as a transcription factor for G1 progression in the cell cycle.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Western
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Cell Cycle / physiology*
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Cell Nucleus / chemistry
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DNA, Neoplasm / analysis
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DNA, Neoplasm / genetics
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DNA-Binding Proteins / analysis
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / physiology
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Electrophoresis, Polyacrylamide Gel
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G1 Phase
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Gene Expression Regulation / genetics*
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Gene Expression Regulation / physiology
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Genes, Homeobox / genetics*
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Homeodomain Proteins*
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Humans
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Immune Sera
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Leukemia-Lymphoma, Adult T-Cell / genetics
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Leukemia-Lymphoma, Adult T-Cell / metabolism
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Leukemia-Lymphoma, Adult T-Cell / pathology
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Molecular Sequence Data
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Nuclear Proteins / genetics*
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Nuclear Proteins / metabolism
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Oncogene Proteins / analysis
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Oncogene Proteins / genetics
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Oncogene Proteins / physiology
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Precipitin Tests
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Proto-Oncogene Mas
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Proto-Oncogene Proteins
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Proto-Oncogenes / genetics*
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RNA, Neoplasm / analysis
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RNA, Neoplasm / genetics
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S Phase
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Transcription Factors / physiology
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Translocation, Genetic / genetics
Substances
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DNA, Neoplasm
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DNA-Binding Proteins
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Homeodomain Proteins
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Immune Sera
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MAS1 protein, human
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Nuclear Proteins
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Oncogene Proteins
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Proto-Oncogene Mas
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Proto-Oncogene Proteins
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RNA, Neoplasm
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Transcription Factors
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TLX1 protein, human