Abstract
Ninety-six multiplex type I diabetic families were typed at the 5' flanking region of the insulin gene by using a PCR assay that better resolves the VNTR into multiple alleles. Affected sibling pairs shared 2, 1, and 0 VNTR alleles--identical by descent--at a frequency of .47, .45, and .08, respectively, a ratio that deviated from the expected 1:2:1 ratio (P < .001). These results confirm linkage of the chromosome 11p15.5 region with type I diabetes mellitus susceptibility.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Age of Onset
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Alleles
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Base Sequence
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Child
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Chromosome Mapping
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Chromosomes, Human, Pair 11*
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Consensus Sequence
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Diabetes Mellitus, Type 1 / genetics*
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Genetic Linkage*
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Genetic Predisposition to Disease
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HLA-DQ Antigens / genetics
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HLA-DR Antigens / genetics*
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Humans
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Insulin / genetics*
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Molecular Sequence Data
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Nuclear Family
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Polymerase Chain Reaction
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Polymorphism, Restriction Fragment Length
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Repetitive Sequences, Nucleic Acid*
Substances
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HLA-DQ Antigens
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HLA-DR Antigens
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Insulin