Enrichment in spontaneous and complement dependent rosette forming cell populations using Ficoll-Hypaque gradient centrifugation

Experientia. 1976 Nov 15;32(11):1484-6. doi: 10.1007/BF01937450.

Abstract

Peripheral blood human T (TL) and B (BL) lymphocytes were separated by continuous Ficoll-Hypaque (FH) gradients. BL were found at the 1050 density interfase (70.5% EAC rosettes) with no TL (0% EAC rosettes); while at the 1068 density interfase there was an enrichment of TL (68% E rosettes) with very few BL (8.5% EAC rosettes).

MeSH terms

  • B-Lymphocytes*
  • Cell Separation / methods
  • Centrifugation, Density Gradient
  • Ficoll
  • Humans
  • Immunologic Techniques
  • T-Lymphocytes*

Substances

  • Ficoll