Abstract
The protein Raf-1, a key mediator of mitogenesis and differentiation, associates with p21ras (refs 1-3). However, the regulation of the serine/threonine kinase activity of Raf-1 is still not understood. Using the yeast two-hybrid system, we identified two structurally related proteins that interact with the aminoterminal region of Raf-1. These proteins, 14-3-3 zeta (PLA2) and 14-3-3 beta (HS1), are members of the 14-3-3 family of proteins. Expression of 14-3-3 proteins in Xenopus oocytes enhanced Raf-1 activity and promoted Raf-1-dependent oocyte maturation. A dominant negative mutant of Raf-1 blocked the effects of 14-3-3 protein.
Publication types
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
14-3-3 Proteins
-
Animals
-
CDC2 Protein Kinase / metabolism
-
Cells, Cultured
-
Enzyme Activation
-
Nerve Tissue Proteins / metabolism*
-
Oocytes
-
Oogenesis / genetics
-
Oogenesis / physiology
-
Protein Serine-Threonine Kinases / metabolism*
-
Proto-Oncogene Proteins / metabolism*
-
Proto-Oncogene Proteins c-raf
-
Recombinant Proteins / metabolism
-
Saccharomyces cerevisiae / genetics
-
Tyrosine 3-Monooxygenase*
-
Xenopus
Substances
-
14-3-3 Proteins
-
Nerve Tissue Proteins
-
Proto-Oncogene Proteins
-
Recombinant Proteins
-
Tyrosine 3-Monooxygenase
-
Protein Serine-Threonine Kinases
-
Proto-Oncogene Proteins c-raf
-
CDC2 Protein Kinase