Abstract
We have quantified mRNA for the hepatocyte growth factor and its putative receptor the c-met proto-oncogene protein product, in a series of human primary and secondary liver tumours and adjacent non-neoplastic liver. In all hepatocellular cancers, hepatocyte growth factor 6 kb mRNA expression was less (mean 23.93% +/- 6.33% S.E.M. n = 7) in the tumours than in the adjacent normal liver. Both relative over- and under-expression of c-met transcripts were found in tumour tissue compared to non-neoplastic liver. Thus hepatocellular cancer tissue does not over-express mRNA for hepatocyte growth factor, though this growth factor might play a role in hyperproliferative states leading to liver cancer.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adenoma / chemistry*
-
Adenoma / genetics
-
Base Sequence
-
Blotting, Northern
-
Carcinoma, Hepatocellular / chemistry*
-
Carcinoma, Hepatocellular / genetics
-
DNA, Neoplasm / analysis
-
DNA, Neoplasm / genetics
-
Gene Expression Regulation, Neoplastic
-
Hepatocyte Growth Factor / analysis
-
Hepatocyte Growth Factor / genetics*
-
Humans
-
Liver / chemistry
-
Liver Neoplasms / chemistry*
-
Liver Neoplasms / genetics
-
Molecular Sequence Data
-
Proto-Oncogene Mas
-
Proto-Oncogene Proteins c-met
-
RNA, Messenger / analysis*
-
RNA, Messenger / genetics
-
Receptor Protein-Tyrosine Kinases / analysis
-
Receptor Protein-Tyrosine Kinases / genetics*
-
Transcription, Genetic
Substances
-
DNA, Neoplasm
-
MAS1 protein, human
-
Proto-Oncogene Mas
-
RNA, Messenger
-
Hepatocyte Growth Factor
-
Proto-Oncogene Proteins c-met
-
Receptor Protein-Tyrosine Kinases