Abstract
A cDNA clone was isolated from HL60 human promyelocyte cells on the basis of the induction of its cognate mRNA by the phorbol ester TPA (12-O-tetradecanoyl phorbol-13-acetate) in the presence of cycloheximide. Northern analysis showed a peak of induction of a 1.9-kb mRNA at 3-6 h after TPA addition. Sequence analysis revealed that this cDNA was likely a human homolog of yeast isopentenyl diphosphate:dimethylallyl diphosphate isomerase, an enzyme important for isoprenoid synthesis and the prenylation of proteins such as ras p21.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Carbon-Carbon Double Bond Isomerases*
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Cell Line
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DNA, Complementary / genetics
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Granulocytes / drug effects
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Granulocytes / metabolism*
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Hemiterpenes
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Humans
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Isomerases / genetics*
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Molecular Sequence Data
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RNA, Messenger / biosynthesis
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RNA, Messenger / genetics*
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / genetics
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Sequence Homology, Amino Acid
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Tetradecanoylphorbol Acetate / pharmacology
Substances
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DNA, Complementary
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Hemiterpenes
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RNA, Messenger
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Isomerases
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Carbon-Carbon Double Bond Isomerases
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isopentenyldiphosphate delta-isomerase
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Tetradecanoylphorbol Acetate