Abstract
A novel human G-protein-coupled seven-transmembrane-type receptor gene, 24-1, has been cloned from THP-1 cells. The 24-1 was 56% and 34% identical to the macrophage inflammatory protein-1 alpha (MIP-1 alpha) receptor and the interleukin-8 (IL-8) receptors at the amino acid level, respectively. To examine the ligand specificity of the receptor, we have established a stable transfectant of this gene with human kidney 293 cells. Monocyte chemoattractant protein-1 (MCP-1) induced a rapid increase of Ca++ influx in the 24-1-transfectant, while other C-C chemokines tested did not. This indicates that the 24-1 encodes the human MCP-1 receptor.
MeSH terms
-
Amino Acid Sequence
-
Calcium / metabolism
-
Cell Line
-
Chemokine CCL2
-
Chemotactic Factors / pharmacology
-
Cloning, Molecular*
-
DNA Probes
-
DNA, Complementary / genetics*
-
GTP-Binding Proteins / physiology
-
Gene Expression*
-
Humans
-
Kidney
-
Molecular Sequence Data
-
Organ Specificity
-
Plasmids
-
Polymerase Chain Reaction
-
RNA, Messenger / metabolism
-
Receptors, CCR2
-
Receptors, Chemokine*
-
Receptors, Cytokine / chemistry
-
Receptors, Cytokine / genetics*
-
Transfection
Substances
-
Chemokine CCL2
-
Chemotactic Factors
-
DNA Probes
-
DNA, Complementary
-
RNA, Messenger
-
Receptors, CCR2
-
Receptors, Chemokine
-
Receptors, Cytokine
-
GTP-Binding Proteins
-
Calcium