Abstract
Proteasomes are the proteolytic complex responsible for major histocompatibility complex (MHC) class I-restricted antigen presentation. Interferon gamma treatment increases expression MHC-encoded LMP2 and LMP7 subunits of the proteasome and decreases expression of two proteasome subunits, named X and Y, which alters the proteolytic specificity of proteasomes. Molecular cloning of complementary DNAs encoding X and Y showed that their proteins are proteasomal subunits with high amino acid similarity to LMP7 and LMP2, respectively. Thus, interferon gamma may induce subunit replacements of X and Y by LMP7 and LMP2, respectively, producing proteasomes perhaps more appropriate for the immunological processing of endogenous antigens.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cloning, Molecular
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Cysteine Endopeptidases*
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DNA, Complementary / genetics
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Down-Regulation*
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Endopeptidases / chemistry
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Endopeptidases / genetics
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Humans
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Interferon-gamma / pharmacology*
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Major Histocompatibility Complex
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Molecular Sequence Data
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Multienzyme Complexes*
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Proteasome Endopeptidase Complex*
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Proteins / chemistry
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Proteins / genetics*
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Proteins / metabolism
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Sequence Homology, Amino Acid
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Tumor Cells, Cultured
Substances
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DNA, Complementary
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Multienzyme Complexes
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Proteins
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LMP-2 protein
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Interferon-gamma
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Endopeptidases
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Cysteine Endopeptidases
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LMP7 protein
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PSMB5 protein, human
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PSMB6 protein, human
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Proteasome Endopeptidase Complex
Associated data
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GENBANK/D29011
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GENBANK/D29012