Abstract
We have used the technique of differential display of mRNA to investigate gene expression patterns in a human squamous carcinoma cell line, SCC-35, following exposure to ionizing radiation. We observed that elongation factor 1 delta (EF-1 delta) expression increased within 2 h of radiation exposure with maximum expression at 8 h. These data identify EF-1 delta as a radiation-inducible gene. Cell cycle analysis shows that EF-1 delta expression enhancement precedes G2 arrest in these cells, suggesting that EF-1 delta may participate in the G2-M cell cycle checkpoint.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Carcinoma, Squamous Cell / genetics*
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Carcinoma, Squamous Cell / metabolism
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Carcinoma, Squamous Cell / pathology
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Cell Cycle
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Gene Expression / radiation effects*
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Head and Neck Neoplasms / genetics*
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Head and Neck Neoplasms / metabolism
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Head and Neck Neoplasms / pathology
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Humans
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Molecular Sequence Data
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Peptide Elongation Factor 1
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Peptide Elongation Factors / genetics
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Peptide Elongation Factors / metabolism
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Peptide Elongation Factors / radiation effects*
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RNA, Messenger / isolation & purification
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RNA, Neoplasm / isolation & purification
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Time Factors
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Tumor Cells, Cultured
Substances
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Peptide Elongation Factor 1
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Peptide Elongation Factors
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RNA, Messenger
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RNA, Neoplasm