The effects of interleukin-10 on interleukin-1 receptor antagonist and interleukin-1 beta production in human monocytes and neutrophils

Lymphokine Cytokine Res. 1994 Feb;13(1):47-54.

Abstract

LPS stimulates human monocytes and neutrophils to produce IL-1 beta and IL-1 receptor antagonist (IL-1ra). IL-10 has been shown to inhibit LPS-induced IL-1 beta production in human monocytes. The objective of these studies was to examine the effects of IL-10 on human monocyte and neutrophil IL-1ra protein and mRNA production and compare it to IL-1 beta. IL-10 markedly inhibited LPS-induced IL-1 beta mRNA and protein production in both cell types. IL-10 alone induced IL-1ra mRNA production in monocytes with a low level of protein production. Furthermore, IL-10 led to enhanced levels of IL-1ra mRNA in LPS-induced monocytes at 2 to 6 h and of IL-1ra protein at 4-16 h, but there was no increase in levels of IL-1ra protein at 24 h or later. In neutrophils IL-10 alone did not stimulate IL-1ra protein or mRNA production and did not augment LPS-induced IL-1ra mRNA. These net effects of IL-10 resulted in an increase in the ratio of IL-1ra to IL-1 beta in both neutrophils and monocytes. The net effects of IL-10 on inflammatory cells may be important in modulation of biologic responses to IL-1.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cells, Cultured
  • Humans
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1 / biosynthesis*
  • Interleukin-1 / genetics
  • Interleukin-10 / pharmacology*
  • Lipopolysaccharides / pharmacology
  • Monocytes / drug effects
  • Monocytes / immunology
  • Monocytes / metabolism
  • Neutrophils / drug effects
  • Neutrophils / immunology
  • Neutrophils / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Interleukin-1 / antagonists & inhibitors
  • Sialoglycoproteins / biosynthesis*
  • Sialoglycoproteins / genetics

Substances

  • IL1RN protein, human
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1
  • Lipopolysaccharides
  • RNA, Messenger
  • Receptors, Interleukin-1
  • Sialoglycoproteins
  • Interleukin-10