Abstract
DNA from tumor tissue and peripheral blood lymphocytes of primary breast cancer patients was screened for the presence of p53 mutations. In DNA from one tumor we found that the histidine codon 193 (CAT) was somatically converted to arginine (CGT). This amino acid residue is highly conserved in many species, thus suggesting that such mutation plays an important role in the loss of wt-p53 function.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Arginine
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Base Sequence
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Breast Neoplasms / genetics*
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Chickens
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Codon
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Conserved Sequence
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DNA Primers
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Exons
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Female
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Gene Amplification*
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Genes, p53*
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Haplorhini
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Histidine
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Humans
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Mice
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Molecular Sequence Data
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Point Mutation*
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Polymerase Chain Reaction
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Rats
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Sequence Homology, Amino Acid
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Trout
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Tumor Suppressor Protein p53 / chemistry
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Tumor Suppressor Protein p53 / genetics*
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Xenopus
Substances
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Codon
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DNA Primers
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Tumor Suppressor Protein p53
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Histidine
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Arginine