Abstract
The Raf-1 protein, a cytoplasmic serine/threonine kinase, plays an important role in signal transduction pathways. In order to examine the role of Raf-1 in human myeloid leukemia, we determined raf-1 mRNA expression by Northern blot analysis in blast cell samples from 27 acute myeloid leukemia (AML) cases and peripheral blood mononuclear cells from six healthy donors. A normal raf-1 transcript size was detected in all cases investigated. However, overexpression of raf-1 mRNA was found in 2 of 27 AML cases, both of which were erythroleukemias (AML, FAB M6).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acute Disease
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Base Sequence
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DNA Primers
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Gene Expression*
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Humans
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Leukemia, Erythroblastic, Acute / blood
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Leukemia, Erythroblastic, Acute / genetics
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Leukemia, Myeloid / blood
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Leukemia, Myeloid / genetics*
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Leukocytes, Mononuclear / metabolism
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Molecular Sequence Data
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Polymerase Chain Reaction
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Protein Serine-Threonine Kinases / biosynthesis*
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Protein Serine-Threonine Kinases / genetics
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Proto-Oncogene Mas
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Proto-Oncogene Proteins / biosynthesis*
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins c-raf
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Proto-Oncogenes*
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RNA, Messenger / biosynthesis
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RNA, Messenger / blood
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Reference Values
Substances
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DNA Primers
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MAS1 protein, human
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Proto-Oncogene Mas
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Proto-Oncogene Proteins
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RNA, Messenger
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Protein Serine-Threonine Kinases
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Proto-Oncogene Proteins c-raf