Abstract
We studied whether it is possible to obtain sufficient mRNA from old paraffin-embedded samples of spleen and bone marrow for reverse transcription and polymerase chain reaction analysis. Spleen tissue from 6 subjects with chronic myelogenous leukemia was studied for rearrangement of BCR and ABL genes. Analysis was successful in 4 cases. These data indicate the feasibility of retrospective analysis of tissue samples of special interest by molecular techniques.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Bone Marrow*
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Female
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Gene Rearrangement / genetics*
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Gene Rearrangement / radiation effects
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Genes, abl / genetics*
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Genes, abl / radiation effects
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Humans
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive / genetics
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Male
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Middle Aged
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Nuclear Warfare
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Paraffin
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Polymerase Chain Reaction*
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RNA, Messenger / genetics*
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RNA, Messenger / radiation effects
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Spleen*
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Time Factors
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Tissue Preservation
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Transcription, Genetic