A novel gene delivery system using EGF receptor-mediated endocytosis

J Chen; S Gamou; A Takayanagi; N Shimizu

doi:10.1016/0014-5793(94)80357-9

A novel gene delivery system using EGF receptor-mediated endocytosis

FEBS Lett. 1994 Jan 31;338(2):167-9. doi: 10.1016/0014-5793(94)80357-9.

Authors

J Chen¹, S Gamou, A Takayanagi, N Shimizu

Affiliation

¹ Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan.

PMID: 8307175
DOI: 10.1016/0014-5793(94)80357-9

Abstract

A monoclonal antibody to the human epidermal growth factor (EGF) receptor was conjugated with polylysine and the resulting conjugate was affinity-linked with DNA (gene). This novel gene delivery system utilizes receptor-mediated endocytosis and would be especially suitable for gene therapy for EGF receptor-overproducing squamous cell carcinomas.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal
Carcinoma, Squamous Cell
Chloramphenicol O-Acetyltransferase / genetics
DNA / administration & dosage
DNA / metabolism
Electrophoresis, Agar Gel
Endocytosis*
ErbB Receptors / physiology*
Gene Transfer Techniques*
Humans
Polylysine
Tumor Cells, Cultured

Substances

Antibodies, Monoclonal
Polylysine
DNA
Chloramphenicol O-Acetyltransferase
ErbB Receptors