Abstract
The presence of parathyroid hormone-related protein (PTHrP) and human papillomavirus (HPV) in a series of gynecological tumors from 131 unselected patients was examined. PTHrP was localized immunohistochemically using a highly specific rabbit polyclonal anti-serum against PTHrP(1-16). The results confirmed that gynecological malignancies, although rarely associated with humoral hypercalcemia of malignancy (HHM), stained for PTHrP in a majority of the squamous-cell carcinomas (SCC) at all sites, but only in a minority of adenocarcinomas, and then in areas of squamous metaplasia. This included a series of endometrial tumors. Detection of HPV types was achieved using a polymerase-chain-reaction (PCR) detection system enabling the detection of HPV types 6, 11, 16, 18, 31, 33 and 45. PTHrP production was not directly related to HPV infection, but correlated with the type of tumor.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / pathology
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Adenocarcinoma / virology
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Base Sequence
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Blotting, Southern
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Carcinoma / microbiology*
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Carcinoma / pathology*
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Carcinoma / therapy
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Carcinoma, Adenosquamous / pathology
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Carcinoma, Adenosquamous / virology
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Carcinoma, Squamous Cell / pathology
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Carcinoma, Squamous Cell / therapy
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Carcinoma, Squamous Cell / virology
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DNA Primers
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DNA, Viral / analysis
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DNA, Viral / genetics
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Endometrial Neoplasms / pathology
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Endometrial Neoplasms / virology
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Female
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Genital Neoplasms, Female / pathology*
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Genital Neoplasms, Female / therapy
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Genital Neoplasms, Female / virology*
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Humans
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Immunohistochemistry
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Molecular Sequence Data
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Neoplasm Staging
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Oligonucleotide Probes
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Papillomaviridae / isolation & purification*
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Parathyroid Hormone-Related Protein*
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Peptide Fragments / analysis*
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Polymerase Chain Reaction / methods
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Proteins / analysis*
Substances
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DNA Primers
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DNA, Viral
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Oligonucleotide Probes
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Parathyroid Hormone-Related Protein
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Peptide Fragments
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Proteins
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parathyroid hormone-related protein (1-16)