Abstract
I have subcloned and sequenced the genes cmtB and cmtA of Escherichia coli K-12 which lie adjacent to the tkt gene on the chromosome. The genes cmtB and cmtA could encode a cytoplasmic protein (EIIA) and an integral-membrane protein (EIIBC), respectively, of the bacterial phospho enol pyruvate-dependent carbohydrate phosphotransferase system. High similarity to the Enzymes IIMtl of Escherichia coli K-12 (gene mtlA) and of Staphylococcus carnosus was detected, but the two genes did not complement mannitol-negative E. coli mutants without the use of a heterologous promoter.
MeSH terms
-
Amino Acid Sequence
-
Base Sequence
-
DNA, Bacterial
-
Escherichia coli / enzymology
-
Escherichia coli / genetics*
-
Escherichia coli Proteins
-
Genes, Bacterial*
-
Molecular Sequence Data
-
Monosaccharide Transport Proteins
-
Open Reading Frames*
-
Phosphoenolpyruvate Sugar Phosphotransferase System / genetics*
-
Restriction Mapping
-
Sequence Homology, Amino Acid
-
Staphylococcus / enzymology
-
Staphylococcus / genetics
-
Transketolase / genetics*
Substances
-
DNA, Bacterial
-
Escherichia coli Proteins
-
Monosaccharide Transport Proteins
-
Transketolase
-
Phosphoenolpyruvate Sugar Phosphotransferase System
-
mannitol PTS permease, E coli
Associated data
-
GENBANK/D12771
-
GENBANK/L04613
-
GENBANK/L04614
-
GENBANK/L04615
-
GENBANK/L04616
-
GENBANK/L17031
-
GENBANK/L17032
-
GENBANK/L17033
-
GENBANK/X61667
-
GENBANK/X72677