Two open reading frames adjacent to the Escherichia coli K-12 transketolase (tkt) gene show high similarity to the mannitol phosphotransferase system enzymes from Escherichia coli and various gram-positive bacteria

Biochim Biophys Acta. 1993 Aug 20;1158(1):103-6. doi: 10.1016/0304-4165(93)90103-f.

Abstract

I have subcloned and sequenced the genes cmtB and cmtA of Escherichia coli K-12 which lie adjacent to the tkt gene on the chromosome. The genes cmtB and cmtA could encode a cytoplasmic protein (EIIA) and an integral-membrane protein (EIIBC), respectively, of the bacterial phospho enol pyruvate-dependent carbohydrate phosphotransferase system. High similarity to the Enzymes IIMtl of Escherichia coli K-12 (gene mtlA) and of Staphylococcus carnosus was detected, but the two genes did not complement mannitol-negative E. coli mutants without the use of a heterologous promoter.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA, Bacterial
  • Escherichia coli / enzymology
  • Escherichia coli / genetics*
  • Escherichia coli Proteins
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Monosaccharide Transport Proteins
  • Open Reading Frames*
  • Phosphoenolpyruvate Sugar Phosphotransferase System / genetics*
  • Restriction Mapping
  • Sequence Homology, Amino Acid
  • Staphylococcus / enzymology
  • Staphylococcus / genetics
  • Transketolase / genetics*

Substances

  • DNA, Bacterial
  • Escherichia coli Proteins
  • Monosaccharide Transport Proteins
  • Transketolase
  • Phosphoenolpyruvate Sugar Phosphotransferase System
  • mannitol PTS permease, E coli

Associated data

  • GENBANK/D12771
  • GENBANK/L04613
  • GENBANK/L04614
  • GENBANK/L04615
  • GENBANK/L04616
  • GENBANK/L17031
  • GENBANK/L17032
  • GENBANK/L17033
  • GENBANK/X61667
  • GENBANK/X72677