Abstract
Vasoactive intestinal peptide (VIP) is a potent mediator of gastrointestinal, nervous, pulmonary, vascular and immune functions. A cDNA was obtained from human HT29 intestinal epithelial cells and found to encode a 457 amino acid, 52 kDa VIP receptor. Transfection of the cDNA into COS-7 cells and 293 cells resulted in expression of specific saturable binding of VIP with a Kd of 0.8 nM, and induction of increases in intracellular cAMP by VIP with an EC50 of 1 nM. The human VIP receptor is homologous to other G protein-coupled receptors of the secretin-parathyroid hormone receptor family. A 2.8 kb transcript was detected in human lung, HT29 cells and Raji B-lymphoblasts with weaker expression in human brain, heart, kidney, liver and placenta.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Line
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Cloning, Molecular
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Cyclic AMP / metabolism
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Gene Expression
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Glioma
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Humans
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Hybrid Cells
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Kinetics
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Mice
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Molecular Sequence Data
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Neuroblastoma
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Oligodeoxyribonucleotides
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Polymerase Chain Reaction / methods
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RNA, Messenger / metabolism*
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Rats
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Receptors, Gastrointestinal Hormone / biosynthesis*
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Receptors, Gastrointestinal Hormone / genetics
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Receptors, Gastrointestinal Hormone / metabolism
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Receptors, Vasoactive Intestinal Peptide
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / metabolism
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Sequence Homology, Amino Acid
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Transfection
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Vasoactive Intestinal Peptide / metabolism*
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Vasoactive Intestinal Peptide / pharmacology
Substances
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Oligodeoxyribonucleotides
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RNA, Messenger
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Receptors, Gastrointestinal Hormone
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Receptors, Vasoactive Intestinal Peptide
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Recombinant Proteins
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Vasoactive Intestinal Peptide
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Cyclic AMP
Associated data
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GENBANK/L13288
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GENBANK/U11079
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GENBANK/U11080
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GENBANK/U11081
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GENBANK/U11082
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GENBANK/U11083
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GENBANK/U11084
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GENBANK/U11085
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GENBANK/U11086
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GENBANK/U11087