Abstract
We have cloned a human receptor for parathyroid hormone from a kidney complementary DNA library. The deduced sequence of 593 amino acids shows high homology to the previously cloned receptors from opossum and rat. Expressed in COS-1 cells, the human receptor binds to parathyroid hormone-(1-38) with high affinity (pKD = 8.5) and is functionally coupled to adenylate cyclase (pEC50 = 9.4). At high concentrations of agonist, the receptor also activates phosphoinositide turnover.
MeSH terms
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Adenylyl Cyclases / metabolism
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Animals
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Base Sequence
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Blotting, Northern
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Cells, Cultured
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Cloning, Molecular
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DNA / biosynthesis
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Humans
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Kidney / enzymology
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Kidney / metabolism
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Molecular Sequence Data
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Parathyroid Hormone / metabolism
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Polymerase Chain Reaction
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Rats
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Receptors, Cell Surface / metabolism*
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Receptors, Parathyroid Hormone
Substances
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Parathyroid Hormone
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Receptors, Cell Surface
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Receptors, Parathyroid Hormone
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DNA
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Adenylyl Cyclases
Associated data
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GENBANK/X66042
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GENBANK/X66043
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GENBANK/X66045
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GENBANK/X66046
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GENBANK/X67167
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GENBANK/X68596
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GENBANK/Z18316
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GENBANK/Z18317
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GENBANK/Z18318
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GENBANK/Z18319