Abstract
We have used the human neuroblastoma cell line SH-SY5Y as a model system to investigate the expression and regulation of the receptors for brain-derived neurotrophic factor (BDNF), a member of the nerve growth factor (NGF) family of neurotrophins. We demonstrate that SH-SY5Y cells express transcripts encoding the low-affinity NGF receptor (LNGFR) and trkB, the signal transducing receptor unit for BDNF. Interaction of BDNF with SH-SY5Y cells increased the transcription of the c-fos gene, showing that these molecules encode functional BDNF receptors. Our findings that differentiating agents such as retinoids and cAMP analogs increased the expression of LNGFR, but decreased trkB mRNA levels, suggest that LNGFR and trkB have different roles during neuronal differentiation.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Base Sequence
-
Brain-Derived Neurotrophic Factor*
-
Bucladesine / pharmacology
-
DNA, Single-Stranded
-
Gene Expression / drug effects
-
Humans
-
Membrane Proteins / genetics*
-
Molecular Sequence Data
-
Nerve Tissue Proteins / metabolism
-
Neuroblastoma
-
Protein-Tyrosine Kinases / genetics*
-
Proto-Oncogene Proteins c-fos / biosynthesis
-
Proto-Oncogene Proteins c-fos / genetics
-
RNA, Messenger / drug effects
-
RNA, Messenger / genetics
-
RNA, Messenger / metabolism*
-
Receptor, Ciliary Neurotrophic Factor
-
Receptors, Nerve Growth Factor / biosynthesis
-
Receptors, Nerve Growth Factor / genetics*
-
Receptors, Nerve Growth Factor / metabolism
-
Retinoids / pharmacology
-
Tumor Cells, Cultured
Substances
-
Brain-Derived Neurotrophic Factor
-
DNA, Single-Stranded
-
Membrane Proteins
-
Nerve Tissue Proteins
-
Proto-Oncogene Proteins c-fos
-
RNA, Messenger
-
Receptor, Ciliary Neurotrophic Factor
-
Receptors, Nerve Growth Factor
-
Retinoids
-
brain-derived growth factor
-
Bucladesine
-
Protein-Tyrosine Kinases