5-lipoxygenase-activating protein is an arachidonate binding protein

FEBS Lett. 1993 Mar 8;318(3):277-81. doi: 10.1016/0014-5793(93)80528-3.

Abstract

5-Lipoxygenase-activating protein (FLAP) is an 18-kDa integral membrane protein which is essential for cellular leukotriene (LT) synthesis, and is the target of LT biosynthesis inhibitors. However, the mechanism by which FLAP activates 5-LO has not been determined. We have expressed high levels of human FLAP in Spodoptera frugiperda (Sf9) insect cells infected with recombinant baculovirus, and used this system to demonstrate that FLAP specifically binds [125I]L-739,059, a novel photoaffinity analog of arachidonic acid. This binding is inhibited by both arachidonic acid and MK-886, an LT biosynthesis inhibitor which specifically interacts with FLAP. These studies suggest that FLAP may activate 5-LO by specifically binding arachidonic acid and transferring this substrate to the enzyme.

MeSH terms

  • 5-Lipoxygenase-Activating Proteins
  • Affinity Labels
  • Animals
  • Arachidonate 5-Lipoxygenase / metabolism
  • Arachidonic Acids / metabolism
  • Baculoviridae / genetics
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Line
  • Enzyme Activation
  • Gene Expression
  • Humans
  • Indoles / metabolism
  • Iodine Radioisotopes / metabolism
  • Membrane Proteins / genetics
  • Membrane Proteins / metabolism*
  • Moths / metabolism
  • Photochemistry
  • Quinolines / metabolism
  • Recombinant Proteins / metabolism
  • Transfection

Substances

  • 5-Lipoxygenase-Activating Proteins
  • ALOX5AP protein, human
  • Affinity Labels
  • Arachidonic Acids
  • Carrier Proteins
  • Indoles
  • Iodine Radioisotopes
  • Membrane Proteins
  • Quinolines
  • Recombinant Proteins
  • L 691831
  • L 739059
  • Arachidonate 5-Lipoxygenase