Abstract
Markers that predict tumor aggressiveness on a case-by-case basis would enable individualization and optimization of oncologic therapy. To achieve this goal, the presence and specific type of K-ras-2 point mutation was determined from formalin-fixed, paraffin-embedded tissue sites in 247 primary and 166 metastatic-recurrent colorectal adenocarcinomas, using a novel approach consisting of topographic tissue selection, DNA amplification, and direct sequencing applicable to large and needle-biopsy-sized specimens. The results provide the basis for a genotypic classification of colorectal cancer capable of predicting individual tumor aggressiveness, including the pattern and extent of metastasis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / genetics
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Adenocarcinoma / pathology*
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Adenocarcinoma / secondary
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Aspartic Acid / genetics
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Biomarkers, Tumor / genetics
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Codon / genetics
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Colonic Neoplasms / genetics*
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Colonic Neoplasms / pathology*
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DNA, Neoplasm / genetics
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Exons / genetics
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Follow-Up Studies
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Genes, ras / genetics*
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Glutamine / genetics
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Histidine / genetics
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Humans
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Mutation / genetics*
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Neoplasm Invasiveness / genetics
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Neoplasm Recurrence, Local
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Neoplasm Staging
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Rectal Neoplasms / genetics*
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Rectal Neoplasms / pathology*
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Retrospective Studies
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Survival Rate
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Valine / genetics
Substances
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Biomarkers, Tumor
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Codon
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DNA, Neoplasm
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Glutamine
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Aspartic Acid
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Histidine
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Valine