p53 expression and K-ras mutation in colorectal adenomas

Gut. 1993 May;34(5):621-4. doi: 10.1136/gut.34.5.621.

Abstract

The frequency of p53 overexpression and K-ras codon 12 mutation was investigated in a series of colorectal adenomas. p53 was detected by immunohistochemistry in only 5% of tumours, whereas K-ras mutation was found in eight of 30 adenomas examined. In vitro, mutant p53 and ras genes cooperate to transform primary rat cells into a tumourigenic cell line. The presence of both p53 overexpression and K-ras mutation in a benign tubulovillous polyp in the present series suggests that in vivo this combination of events is insufficient to cause malignant transformation of a large bowel adenoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoma / chemistry
  • Adenoma / genetics*
  • Colorectal Neoplasms / chemistry
  • Colorectal Neoplasms / genetics*
  • Genes, p53 / genetics*
  • Genes, ras / genetics*
  • Humans
  • Immunohistochemistry
  • Intestinal Polyps / chemistry
  • Intestinal Polyps / genetics
  • Mutation / genetics*
  • Tumor Suppressor Protein p53 / analysis*

Substances

  • Tumor Suppressor Protein p53