Genes on chromosomes 4, 9, and 19 involved in 11q23 abnormalities in acute leukemia share sequence homology and/or common motifs

Proc Natl Acad Sci U S A. 1993 May 15;90(10):4631-5. doi: 10.1073/pnas.90.10.4631.

Abstract

Chromosome translocations involving band 11q23 are associated with human acute leukemias. These translocations fuse the ALL-1 gene, homolog of Drosophila trithorax and located at chromosome band 11q23, to genes from a variety of chromosomes. We cloned and sequenced cDNAs derived from transcripts of the AF-4 and AF-9 genes involved in the most common chromosome abnormalities, t(4:11)(q21:q23) and t(9:11)(p22:q23), respectively. Sequence analysis indicates high homology between the AF-9 gene protein product and the protein encoded by the ENL gene fused to ALL-1 in (11:19) chromosome translocations. AF-4, AF-9, and ENL proteins contain nuclear targeting sequences as well as serine-rich and proline-rich regions. Stretches abundant in basic amino acids are also present in the three proteins. These results suggest that the different proteins fused to ALL-1 polypeptide(s) provide similar functional domains.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acute Disease
  • Amino Acid Sequence
  • Base Sequence
  • Chromosome Aberrations / genetics*
  • Chromosome Disorders
  • Chromosomes, Human, Pair 11*
  • Chromosomes, Human, Pair 19*
  • Chromosomes, Human, Pair 4*
  • Chromosomes, Human, Pair 9*
  • Cloning, Molecular
  • DNA / genetics
  • DNA, Neoplasm / genetics
  • DNA-Binding Proteins / genetics
  • Genes
  • Humans
  • Leukemia / genetics*
  • Molecular Sequence Data
  • Neoplasm Proteins*
  • Nuclear Proteins / genetics*
  • Oligodeoxyribonucleotides / chemistry
  • Recombinant Fusion Proteins / genetics
  • Restriction Mapping
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Transcription Factors*
  • Transcriptional Elongation Factors
  • Translocation, Genetic

Substances

  • DNA, Neoplasm
  • DNA-Binding Proteins
  • MLLT1 protein, human
  • MLLT3 protein, human
  • Neoplasm Proteins
  • Nuclear Proteins
  • Oligodeoxyribonucleotides
  • Recombinant Fusion Proteins
  • Transcription Factors
  • Transcriptional Elongation Factors
  • AFF1 protein, human
  • DNA

Associated data

  • GENBANK/L13743
  • GENBANK/L13744
  • GENBANK/L13773