Abstract
Chromosome translocations involving band 11q23 are associated with human acute leukemias. These translocations fuse the ALL-1 gene, homolog of Drosophila trithorax and located at chromosome band 11q23, to genes from a variety of chromosomes. We cloned and sequenced cDNAs derived from transcripts of the AF-4 and AF-9 genes involved in the most common chromosome abnormalities, t(4:11)(q21:q23) and t(9:11)(p22:q23), respectively. Sequence analysis indicates high homology between the AF-9 gene protein product and the protein encoded by the ENL gene fused to ALL-1 in (11:19) chromosome translocations. AF-4, AF-9, and ENL proteins contain nuclear targeting sequences as well as serine-rich and proline-rich regions. Stretches abundant in basic amino acids are also present in the three proteins. These results suggest that the different proteins fused to ALL-1 polypeptide(s) provide similar functional domains.
Publication types
-
Comparative Study
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Acute Disease
-
Amino Acid Sequence
-
Base Sequence
-
Chromosome Aberrations / genetics*
-
Chromosome Disorders
-
Chromosomes, Human, Pair 11*
-
Chromosomes, Human, Pair 19*
-
Chromosomes, Human, Pair 4*
-
Chromosomes, Human, Pair 9*
-
Cloning, Molecular
-
DNA / genetics
-
DNA, Neoplasm / genetics
-
DNA-Binding Proteins / genetics
-
Genes
-
Humans
-
Leukemia / genetics*
-
Molecular Sequence Data
-
Neoplasm Proteins*
-
Nuclear Proteins / genetics*
-
Oligodeoxyribonucleotides / chemistry
-
Recombinant Fusion Proteins / genetics
-
Restriction Mapping
-
Sequence Alignment
-
Sequence Homology, Amino Acid
-
Transcription Factors*
-
Transcriptional Elongation Factors
-
Translocation, Genetic
Substances
-
DNA, Neoplasm
-
DNA-Binding Proteins
-
MLLT1 protein, human
-
MLLT3 protein, human
-
Neoplasm Proteins
-
Nuclear Proteins
-
Oligodeoxyribonucleotides
-
Recombinant Fusion Proteins
-
Transcription Factors
-
Transcriptional Elongation Factors
-
AFF1 protein, human
-
DNA
Associated data
-
GENBANK/L13743
-
GENBANK/L13744
-
GENBANK/L13773